Production, purification and diagnostic application of filarial recombinant protein WbSXP-1 expressed in salt inducible Escherichia coli.
Identifieur interne : 007141 ( Main/Exploration ); précédent : 007140; suivant : 007142Production, purification and diagnostic application of filarial recombinant protein WbSXP-1 expressed in salt inducible Escherichia coli.
Auteurs : S. Janardhan [Inde] ; P. Pandiaraja ; S. Thirugnanam ; M N Balamurali ; Kennedy Fernando ; H C Mody ; P K Desai ; S. Meenakshisundaram ; P. KalirajSource :
- Journal of industrial microbiology & biotechnology [ 1367-5435 ] ; 2007.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antihelminthe (immunologie), Antigènes d'helminthe (biosynthèse), Antigènes d'helminthe (génétique), Antigènes d'helminthe (immunologie), Bioréacteurs, Chlorure de sodium (pharmacologie), Escherichia coli (), Escherichia coli (génétique), Escherichia coli (métabolisme), Filariose lymphatique (diagnostic), Filariose lymphatique (parasitologie), Filtration (instrumentation), Protéines d'helminthes (immunologie), Protéines d'helminthes (isolement et purification), Protéines recombinantes (biosynthèse), Protéines recombinantes (génétique), Protéines recombinantes (isolement et purification), Sensibilité et spécificité, Wuchereria bancrofti (génétique), Wuchereria bancrofti (immunologie).
- MESH :
- biosynthèse : Antigènes d'helminthe, Protéines recombinantes.
- diagnostic : Filariose lymphatique.
- génétique : Antigènes d'helminthe, Escherichia coli, Protéines recombinantes, Wuchereria bancrofti.
- immunologie : Anticorps antihelminthe, Antigènes d'helminthe, Protéines d'helminthes, Wuchereria bancrofti.
- isolement et purification : Protéines d'helminthes, Protéines recombinantes.
- métabolisme : Escherichia coli.
- parasitologie : Filariose lymphatique.
- pharmacologie : Chlorure de sodium.
- Animaux, Bioréacteurs, Escherichia coli, Filtration, Sensibilité et spécificité.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (immunology), Antigens, Helminth (biosynthesis), Antigens, Helminth (genetics), Antigens, Helminth (immunology), Bioreactors, Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (parasitology), Escherichia coli (drug effects), Escherichia coli (genetics), Escherichia coli (metabolism), Filtration (instrumentation), Helminth Proteins (immunology), Helminth Proteins (isolation & purification), Recombinant Proteins (biosynthesis), Recombinant Proteins (genetics), Recombinant Proteins (isolation & purification), Sensitivity and Specificity, Sodium Chloride (pharmacology), Wuchereria bancrofti (genetics), Wuchereria bancrofti (immunology).
- MESH :
- chemical , biosynthesis : Antigens, Helminth, Recombinant Proteins.
- chemical , genetics : Antigens, Helminth, Recombinant Proteins.
- chemical , immunology : Antibodies, Helminth, Antigens, Helminth, Helminth Proteins.
- diagnosis : Elephantiasis, Filarial.
- drug effects : Escherichia coli.
- genetics : Escherichia coli, Wuchereria bancrofti.
- immunology : Wuchereria bancrofti.
- instrumentation : Filtration.
- chemical , isolation & purification : Helminth Proteins, Recombinant Proteins.
- metabolism : Escherichia coli.
- parasitology : Elephantiasis, Filarial.
- chemical , pharmacology : Sodium Chloride.
- Animals, Bioreactors, Sensitivity and Specificity.
Abstract
Wuchereria bancrofti protein WbSXP-1 was identified and established as a potential candidate for the diagnosis of lymphatic filariasis. For the economic production of rWbSXP-1, osmotically (salt) inducible Escherichia coli GJ1158 was preferred. Cultivation and expression was optimized in 3 L airlift bioreactor (ALB) and was successfully extended to 30 L ALB. Purification of rWbSXP-1 his-tag protein was optimized in technical scale using FPLC and the maximal recovery of rWbSXP-1 with significant level of purity was achieved using the combination of IMAC and gel filtration. Quality criteria for immuno-reactivity of purified rWbSXP-1 were established for diagnostic applications. Enhancement of sensitivity in rapid diagnostic format was optimized to effectively detect weak to strong antibody reactivity in individuals exposed to lymphatic filariasis. Performance of the rapid format during field evaluation was successful. The accelerated stability assessment of the rapid format satisfied the requirements of WHO-cGMP norms. This investigation presents a successful technical scale production and purification of rWbSXP-1 considering the future industrial application and an enhanced rapid flow through antibody assay for the diagnosis of human lymphatic filariasis.
DOI: 10.1007/s10295-007-0243-3
PubMed: 17674066
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 003573
- to stream PubMed, to step Curation: 003573
- to stream PubMed, to step Checkpoint: 003573
- to stream Ncbi, to step Merge: 002826
- to stream Ncbi, to step Curation: 002826
- to stream Ncbi, to step Checkpoint: 002826
- to stream Main, to step Merge: 007269
- to stream Main, to step Curation: 007141
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Production, purification and diagnostic application of filarial recombinant protein WbSXP-1 expressed in salt inducible Escherichia coli.</title><author><name sortKey="Janardhan, S" sort="Janardhan, S" uniqKey="Janardhan S" first="S" last="Janardhan">S. Janardhan</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Biotechnology, Anna University, Chennai, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Biotechnology, Anna University, Chennai</wicri:regionArea><wicri:noRegion>Chennai</wicri:noRegion></affiliation></author><author><name sortKey="Pandiaraja, P" sort="Pandiaraja, P" uniqKey="Pandiaraja P" first="P" last="Pandiaraja">P. Pandiaraja</name></author><author><name sortKey="Thirugnanam, S" sort="Thirugnanam, S" uniqKey="Thirugnanam S" first="S" last="Thirugnanam">S. Thirugnanam</name></author><author><name sortKey="Balamurali, M N" sort="Balamurali, M N" uniqKey="Balamurali M" first="M N" last="Balamurali">M N Balamurali</name></author><author><name sortKey="Fernando, Kennedy" sort="Fernando, Kennedy" uniqKey="Fernando K" first="Kennedy" last="Fernando">Kennedy Fernando</name></author><author><name sortKey="Mody, H C" sort="Mody, H C" uniqKey="Mody H" first="H C" last="Mody">H C Mody</name></author><author><name sortKey="Desai, P K" sort="Desai, P K" uniqKey="Desai P" first="P K" last="Desai">P K Desai</name></author><author><name sortKey="Meenakshisundaram, S" sort="Meenakshisundaram, S" uniqKey="Meenakshisundaram S" first="S" last="Meenakshisundaram">S. Meenakshisundaram</name></author><author><name sortKey="Kaliraj, P" sort="Kaliraj, P" uniqKey="Kaliraj P" first="P" last="Kaliraj">P. Kaliraj</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17674066</idno><idno type="pmid">17674066</idno><idno type="doi">10.1007/s10295-007-0243-3</idno><idno type="wicri:Area/PubMed/Corpus">003573</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003573</idno><idno type="wicri:Area/PubMed/Curation">003573</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003573</idno><idno type="wicri:Area/PubMed/Checkpoint">003573</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003573</idno><idno type="wicri:Area/Ncbi/Merge">002826</idno><idno type="wicri:Area/Ncbi/Curation">002826</idno><idno type="wicri:Area/Ncbi/Checkpoint">002826</idno><idno type="wicri:doubleKey">1367-5435:2007:Janardhan S:production:purification:and</idno><idno type="wicri:Area/Main/Merge">007269</idno><idno type="wicri:Area/Main/Curation">007141</idno><idno type="wicri:Area/Main/Exploration">007141</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Production, purification and diagnostic application of filarial recombinant protein WbSXP-1 expressed in salt inducible Escherichia coli.</title><author><name sortKey="Janardhan, S" sort="Janardhan, S" uniqKey="Janardhan S" first="S" last="Janardhan">S. Janardhan</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Biotechnology, Anna University, Chennai, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Biotechnology, Anna University, Chennai</wicri:regionArea><wicri:noRegion>Chennai</wicri:noRegion></affiliation></author><author><name sortKey="Pandiaraja, P" sort="Pandiaraja, P" uniqKey="Pandiaraja P" first="P" last="Pandiaraja">P. Pandiaraja</name></author><author><name sortKey="Thirugnanam, S" sort="Thirugnanam, S" uniqKey="Thirugnanam S" first="S" last="Thirugnanam">S. Thirugnanam</name></author><author><name sortKey="Balamurali, M N" sort="Balamurali, M N" uniqKey="Balamurali M" first="M N" last="Balamurali">M N Balamurali</name></author><author><name sortKey="Fernando, Kennedy" sort="Fernando, Kennedy" uniqKey="Fernando K" first="Kennedy" last="Fernando">Kennedy Fernando</name></author><author><name sortKey="Mody, H C" sort="Mody, H C" uniqKey="Mody H" first="H C" last="Mody">H C Mody</name></author><author><name sortKey="Desai, P K" sort="Desai, P K" uniqKey="Desai P" first="P K" last="Desai">P K Desai</name></author><author><name sortKey="Meenakshisundaram, S" sort="Meenakshisundaram, S" uniqKey="Meenakshisundaram S" first="S" last="Meenakshisundaram">S. Meenakshisundaram</name></author><author><name sortKey="Kaliraj, P" sort="Kaliraj, P" uniqKey="Kaliraj P" first="P" last="Kaliraj">P. Kaliraj</name></author></analytic><series><title level="j">Journal of industrial microbiology & biotechnology</title><idno type="ISSN">1367-5435</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Helminth (immunology)</term><term>Antigens, Helminth (biosynthesis)</term><term>Antigens, Helminth (genetics)</term><term>Antigens, Helminth (immunology)</term><term>Bioreactors</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (parasitology)</term><term>Escherichia coli (drug effects)</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Filtration (instrumentation)</term><term>Helminth Proteins (immunology)</term><term>Helminth Proteins (isolation & purification)</term><term>Recombinant Proteins (biosynthesis)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (isolation & purification)</term><term>Sensitivity and Specificity</term><term>Sodium Chloride (pharmacology)</term><term>Wuchereria bancrofti (genetics)</term><term>Wuchereria bancrofti (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antihelminthe (immunologie)</term><term>Antigènes d'helminthe (biosynthèse)</term><term>Antigènes d'helminthe (génétique)</term><term>Antigènes d'helminthe (immunologie)</term><term>Bioréacteurs</term><term>Chlorure de sodium (pharmacologie)</term><term>Escherichia coli ()</term><term>Escherichia coli (génétique)</term><term>Escherichia coli (métabolisme)</term><term>Filariose lymphatique (diagnostic)</term><term>Filariose lymphatique (parasitologie)</term><term>Filtration (instrumentation)</term><term>Protéines d'helminthes (immunologie)</term><term>Protéines d'helminthes (isolement et purification)</term><term>Protéines recombinantes (biosynthèse)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (isolement et purification)</term><term>Sensibilité et spécificité</term><term>Wuchereria bancrofti (génétique)</term><term>Wuchereria bancrofti (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antigens, Helminth</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Helminth</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Helminth</term><term>Antigens, Helminth</term><term>Helminth Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Antigènes d'helminthe</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes d'helminthe</term><term>Escherichia coli</term><term>Protéines recombinantes</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Antigènes d'helminthe</term><term>Protéines d'helminthes</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Filtration</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Helminth Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines d'helminthes</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Chlorure de sodium</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Sodium Chloride</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Bioreactors</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Bioréacteurs</term><term>Escherichia coli</term><term>Filtration</term><term>Sensibilité et spécificité</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Wuchereria bancrofti protein WbSXP-1 was identified and established as a potential candidate for the diagnosis of lymphatic filariasis. For the economic production of rWbSXP-1, osmotically (salt) inducible Escherichia coli GJ1158 was preferred. Cultivation and expression was optimized in 3 L airlift bioreactor (ALB) and was successfully extended to 30 L ALB. Purification of rWbSXP-1 his-tag protein was optimized in technical scale using FPLC and the maximal recovery of rWbSXP-1 with significant level of purity was achieved using the combination of IMAC and gel filtration. Quality criteria for immuno-reactivity of purified rWbSXP-1 were established for diagnostic applications. Enhancement of sensitivity in rapid diagnostic format was optimized to effectively detect weak to strong antibody reactivity in individuals exposed to lymphatic filariasis. Performance of the rapid format during field evaluation was successful. The accelerated stability assessment of the rapid format satisfied the requirements of WHO-cGMP norms. This investigation presents a successful technical scale production and purification of rWbSXP-1 considering the future industrial application and an enhanced rapid flow through antibody assay for the diagnosis of human lymphatic filariasis.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Balamurali, M N" sort="Balamurali, M N" uniqKey="Balamurali M" first="M N" last="Balamurali">M N Balamurali</name><name sortKey="Desai, P K" sort="Desai, P K" uniqKey="Desai P" first="P K" last="Desai">P K Desai</name><name sortKey="Fernando, Kennedy" sort="Fernando, Kennedy" uniqKey="Fernando K" first="Kennedy" last="Fernando">Kennedy Fernando</name><name sortKey="Kaliraj, P" sort="Kaliraj, P" uniqKey="Kaliraj P" first="P" last="Kaliraj">P. Kaliraj</name><name sortKey="Meenakshisundaram, S" sort="Meenakshisundaram, S" uniqKey="Meenakshisundaram S" first="S" last="Meenakshisundaram">S. Meenakshisundaram</name><name sortKey="Mody, H C" sort="Mody, H C" uniqKey="Mody H" first="H C" last="Mody">H C Mody</name><name sortKey="Pandiaraja, P" sort="Pandiaraja, P" uniqKey="Pandiaraja P" first="P" last="Pandiaraja">P. Pandiaraja</name><name sortKey="Thirugnanam, S" sort="Thirugnanam, S" uniqKey="Thirugnanam S" first="S" last="Thirugnanam">S. Thirugnanam</name></noCountry><country name="Inde"><noRegion><name sortKey="Janardhan, S" sort="Janardhan, S" uniqKey="Janardhan S" first="S" last="Janardhan">S. Janardhan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 007141 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 007141 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:17674066
|texte= Production, purification and diagnostic application of filarial recombinant protein WbSXP-1 expressed in salt inducible Escherichia coli.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:17674066" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |